diff --git a/Content.Server/GameObjects/Components/AI/AiFactionPrototype.cs b/Content.Server/GameObjects/Components/AI/AiFactionPrototype.cs
new file mode 100644
index 0000000000..1a1a762b5b
--- /dev/null
+++ b/Content.Server/GameObjects/Components/AI/AiFactionPrototype.cs
@@ -0,0 +1,27 @@
+#nullable enable
+using System.Collections.Generic;
+using Robust.Shared.Prototypes;
+using Robust.Shared.Serialization;
+using YamlDotNet.RepresentationModel;
+
+namespace Content.Server.GameObjects.Components.AI
+{
+    [Prototype("aiFaction")]
+    public class AiFactionPrototype : IIndexedPrototype, IPrototype
+    {
+        // These are immutable so any dynamic changes aren't saved back over.
+        // AiFactionSystem will just read these and then store them.
+
+        public string ID { get; private set; } = default!;
+
+        public IReadOnlyList<string> Hostile { get; private set; } = default!;
+
+        public void LoadFrom(YamlMappingNode mapping)
+        {
+            var serializer = YamlObjectSerializer.NewReader(mapping);
+
+            serializer.DataField(this, x => x.ID, "id", string.Empty);
+            serializer.DataField(this, x => x.Hostile, "hostile", new List<string>());
+        }
+    }
+}
diff --git a/Content.Server/GameObjects/Components/AI/AiFactionTagComponent.cs b/Content.Server/GameObjects/Components/AI/AiFactionTagComponent.cs
index 146a7d52ed..8a87590260 100644
--- a/Content.Server/GameObjects/Components/AI/AiFactionTagComponent.cs
+++ b/Content.Server/GameObjects/Components/AI/AiFactionTagComponent.cs
@@ -1,5 +1,7 @@
+#nullable enable
 using System;
 using System.Collections.Generic;
+using Content.Server.GameObjects.EntitySystems.AI;
 using Robust.Shared.GameObjects;
 using Robust.Shared.Serialization;
 
@@ -35,15 +37,4 @@ namespace Content.Server.GameObjects.Components.AI
                 });
         }
     }
-
-    [Flags]
-    public enum Faction
-    {
-        None = 0,
-        NanoTrasen = 1 << 0,
-        SimpleHostile = 1 << 1,
-        SimpleNeutral = 1 << 2,
-        Syndicate = 1 << 3,
-        Xeno = 1 << 4,
-    }
 }
diff --git a/Content.Server/GameObjects/EntitySystems/AI/AiFactionTagSystem.cs b/Content.Server/GameObjects/EntitySystems/AI/AiFactionTagSystem.cs
index 9e6b06a4b7..2602b09a09 100644
--- a/Content.Server/GameObjects/EntitySystems/AI/AiFactionTagSystem.cs
+++ b/Content.Server/GameObjects/EntitySystems/AI/AiFactionTagSystem.cs
@@ -1,6 +1,11 @@
+#nullable enable
+using System;
 using System.Collections.Generic;
 using Content.Server.GameObjects.Components.AI;
 using Robust.Shared.GameObjects;
+using Robust.Shared.IoC;
+using Robust.Shared.Log;
+using Robust.Shared.Prototypes;
 
 namespace Content.Server.GameObjects.EntitySystems.AI
 {
@@ -14,27 +19,44 @@ namespace Content.Server.GameObjects.EntitySystems.AI
          *    This may change where specified friendly factions are listed. (e.g. to get number of friendlies in area).
          */
 
+        private readonly Dictionary<Faction, Faction> _hostileFactions = new();
+
+        public override void Initialize()
+        {
+            base.Initialize();
+            var protoManager = IoCManager.Resolve<IPrototypeManager>();
+
+            foreach (var faction in protoManager.EnumeratePrototypes<AiFactionPrototype>())
+            {
+                if (Enum.TryParse(faction.ID,  out Faction @enum))
+                {
+                    var parsedFaction = Faction.None;
+
+                    foreach (var hostile in faction.Hostile)
+                    {
+                        if (Enum.TryParse(hostile, out Faction parsedHostile))
+                        {
+                            parsedFaction |= parsedHostile;
+                        }
+                        else
+                        {
+                            Logger.Error($"Unable to parse hostile faction {hostile} for {faction.ID}");
+                        }
+                    }
+
+                    _hostileFactions[@enum] = parsedFaction;
+                }
+                else
+                {
+                    Logger.Error($"Unable to parse AI faction {faction.ID}");
+                }
+            }
+        }
+
         public Faction GetHostileFactions(Faction faction) => _hostileFactions.TryGetValue(faction, out var hostiles) ? hostiles : Faction.None;
 
-        private readonly Dictionary<Faction, Faction> _hostileFactions = new()
-        {
-            {Faction.NanoTrasen,
-                Faction.SimpleHostile | Faction.Syndicate | Faction.Xeno},
-            {Faction.SimpleHostile,
-                Faction.NanoTrasen | Faction.Syndicate
-            },
-            // What makes a man turn neutral?
-            {Faction.SimpleNeutral,
-                Faction.None
-            },
-            {Faction.Syndicate,
-                Faction.NanoTrasen | Faction.SimpleHostile | Faction.Xeno},
-            {Faction.Xeno,
-                Faction.NanoTrasen | Faction.Syndicate},
-        };
-
         public Faction GetFactions(IEntity entity) =>
-            entity.TryGetComponent(out AiFactionTagComponent factionTags)
+            entity.TryGetComponent(out AiFactionTagComponent? factionTags)
             ? factionTags.Factions
             : Faction.None;
 
@@ -83,4 +105,15 @@ namespace Content.Server.GameObjects.EntitySystems.AI
             _hostileFactions[source] = hostileFactions;
         }
     }
+
+    [Flags]
+    public enum Faction
+    {
+        None = 0,
+        NanoTrasen = 1 << 0,
+        SimpleHostile = 1 << 1,
+        SimpleNeutral = 1 << 2,
+        Syndicate = 1 << 3,
+        Xeno = 1 << 4,
+    }
 }
diff --git a/Resources/Prototypes/ai_factions.yml b/Resources/Prototypes/ai_factions.yml
new file mode 100644
index 0000000000..1a60f55763
--- /dev/null
+++ b/Resources/Prototypes/ai_factions.yml
@@ -0,0 +1,28 @@
+- type: aiFaction
+  id: NanoTrasen
+  hostile:
+  - SimpleHostile
+  - Syndicate
+  - Xeno
+
+- type: aiFaction
+  id: SimpleHostile
+  hostile:
+  - NanoTrasen
+  - Syndicate
+
+- type: aiFaction
+  id: SimpleNeutral
+
+- type: aiFaction
+  id: Syndicate
+  hostile:
+  - NanoTrasen
+  - SimpleHostile
+  - Xeno
+
+- type: aiFaction
+  id: Xeno
+  hostile:
+  - NanoTrasen
+  - Syndicate